This study investigated timber rattlesnake movement and spatial use of the landscape, population statistics, and thermal habitat in landscapes being managed with prescribed fire. Results indicate that timber rattlesnakes typically do not use habitat that was recently burned. It appears that timber rattlesnakes in northeastern Pennsylvania may be able to avoid the direct impacts of prescribed fire (i.e., injury and mortality). However, this is likely to be different from one prescribed fire to another. Although burned landscapes may have no negative direct impact on timber rattlesnakes, the inability to obtain chemical stimuli may make these habitats unpreferred.