The buffalo nut (Pyrularia pubera Michx.; Cervantesiaceae) is an unusual, dioecious (male or female)
small forest tree that is able to grow on its own, or more often, as a root parasite of other plants. Native
to the Appalachian mountain range, the buffalo nut is at its northern-most limit in western
Pennsylvania, where it was formerly reputed to be common, but now is listed as rare. Only two
populations are in protected areas, one at Powdermill Nature Reserve and one at Ohiopyle State Park.
This study aspired to establish whether or not remaining populations are comprised of different
individuals or mostly clones, the degree of genetic variation among remaining Pennsylvania populations,
and how the Pennsylvania populations relate to the closest remaining populations in Virginia, West
Virginia, and Kentucky. In addition, the effectiveness of sexual reproduction in remaining populations
was explored.